Coding
Cry4Ba

Part:BBa_K5311003:Design

Designed by: Carlos Perez de Arenaza, Jose Luis Perez and Oriana Castillo   Group: iGEM24_UPF-Barcelona   (2024-09-24)


CDS Cry4Ba toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1531
    Illegal BglII site found at 2761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 293
    Illegal NgoMIV site found at 3252
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1362
    Illegal BsaI.rc site found at 3465


Design Notes

This sequence was codon optimized for Cutibacterium acnes


Source

You can find the source for this part by using the following link: https://www.uniprot.org/uniprotkb/P05519/entry#sequences or entering to uniprot.org and introducing the following code: P05519

References

[1] Boonserm, P., Davis, P., Ellar, D. J., & Li, J. (2005). Crystal Structure of the Mosquito-larvicidal Toxin Cry4Ba and Its Biological Implications. Journal Of Molecular Biology, 348(2), 363-382. https://doi.org/10.1016/j.jmb.2005.02.013